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. 2009 Mar 13;5(3):e1000304. doi: 10.1371/journal.pcbi.1000304

Figure 3. Study of interactions between fragments of huntingtin.

Figure 3

(A) Schematic overview of huntingtin fragments used in Y2H and LUMIER experiments. (B) The results obtained with the Y2H assays. (C) The expression of different fusion pairs was analyzed by Western blot using antibodies against V5-epitope (Invitrogen, 1∶5000, monoclonal antibody) and Protein-A (Sigma 1∶2000, polyclonal antibody); 15 µl from 100 µl of each cell extract was loaded onto SDS-PAGE gel. Detection with anti-tubulin antibodies was used as a loading control. (D) Firefly luciferase activities of immunopurified protein complexes in relative fluorescence units (RFU).