Skip to main content
. 2009 Feb 19;28(7):889–901. doi: 10.1038/emboj.2009.29

Figure 1.

Figure 1

(A) Immunoblot analysis of BAG1, BAG3, Hsc/Hsp70 and Hsp90 in young and old I90 cells. For detection of BAG proteins, an antibody directed against the conserved BAG domain was used. (B) Real-time PCR analysis of human BAG family members and Hsc70 and Hsp90 in young and old I90 cells. Depicted is the expression ratio (log2) of target genes in old cells relative to young cells. *P<0.05 and **P<0.01 versus young, n=3. (C) Co-IP analysis of Hsc/Hsp70 interaction with BAG1 and BAG3 in young (Y) and old (O) I90 cells. Upper panel shows relative amounts of proteins in cell lysates used for Co-IP (Input). Middle panel shows levels of BAG1 and BAG3 found in Hsc/Hsp70 immunoprecipitates. Lower panel shows levels of Hsc/Hsp70 co-sedimented upon immunoprecipitation of BAG3. (D, E) 293 cells were treated with the indicated agents for 8 h followed by immunoblot analysis of indicated proteins.