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. 2009 Feb 19;28(7):889–901. doi: 10.1038/emboj.2009.29

Figure 7.

Figure 7

(A) Protein extracts from cerebellum (CER) of young (Y, 3 months) and old (O, 24 months) mice were analysed for BAG1 and BAG3 expression by immunoblot analysis. (B) Real-time PCR analysis of indicated mRNA levels in cerebellum of young and old mice. Depicted is the log2 expression ratio of target genes in old mice relative to young mice. Values are expressed as mean±s.e.m. *P<0.05 and **P<0.01 versus young, n=3. (C) Immunoblot analysis as in (A) but in cortex (CTX), hippocampus (HIP) and mid-brain (MB). (D) Western-blot analysis of indicated proteins in different brain regions of young and old mice. (E) Total cathepsin and specific cathepsin B activity in brain extracts from young and old mice was determined using fluorescence probes Z-FR-AMC and Z-RR-AMC, respectively, as described in the Material and methods section. Values are expressed as mean±s.e.m. *P<0.05 versus young, n=3. (F) Expression analysis of indicated proteins in primary hippocampal astrocytic and neuronal cell cultures from young (2 months) and old (24 months) rats. Detection of GLT1 and NeuN served as astrocyte and neuron markers, respectively. Note the NeuN signals in astrocytic cultures. We attributed these signals to the presence of resting nuclei of dead neurons, which we observed under the microscope.