Skip to main content
. Author manuscript; available in PMC: 2009 Feb 25.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2007 Apr 5;27(6):1447–1455. doi: 10.1161/ATVBAHA.107.143438

Figure 4.

Figure 4

Membrane cholesterol depletion/modification impairs TF-VIIa—induced IL-8 gene expression. Monolayers of MDA-MB-231 cells were treated with varying concentrations of mβCD (A and B) or filipin (C and D), and then stimulated with VIIa (10 nM) for 90 minutes. Total RNA was extracted and subjected to Northern blot analysis to probe IL-8 gene expression. Data shown in (A) and (C) represent mean±SEM from 4 to 6 experiments, and representative Northern blots were shown in (B) and (D). *Significantly (P<0.05) differs form the value obtained with control (not treated with mβCD or filipin) cells exposed to VIIa.