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. Author manuscript; available in PMC: 2009 Feb 25.
Published in final edited form as: Cell Microbiol. 2008 Jan 7;10(5):1153–1165. doi: 10.1111/j.1462-5822.2008.01117.x

Fig 3.

Fig 3

RT-PCR analysis of cyclin expression in response to EGF or T. gondii infection. HFF were left untreated (NT), stimulated with EGF (20ng/ml), or infected with T. gondii RH at an m.o.i. of 5:1. RNA was isolated after 8, 12, 24, and 32 hours of incubation. RT-PCR amplification and analysis of gene expression of cyclin D1 (A), cyclin E1 (B), cyclin E2 (C), cyclin A (D), cyclin B (E), and β-actin (F) were performed as described in Experimental Procedures. Gel images and corresponding densitometric profiles are shown from a representative experiment of three performed. Increased steady state message levels are observed following infection and EGF treatment consistent with cell cycle entry.