Fig. 7.

Infection with replication-deficient parasites causes a moderate abrogation in the levels of cyclins coupled to a failure in the sustained activation of ERK. Confluent HFF were infected with T. gondii RH at an m.o.i. of 5:1 in the absence (A) or presence of 10 μM pyrimethamine (B) for the indicated time points. Cells were also infected at an equivalent m.o.i. with parasites that had emerged from one growth cycle under 10 μM clindamycin treatment and incubated in media free of antibiotics (C). Infected cells were processed for SDS-PAGE followed by immunoblotting using antibodies against cyclin D₁, cyclin E₁, cyclin A, β-actin, phosphorylated ERK and total ERK. Results correspond to one representative experiment of three performed. PYR, pyrimethamine; CLIND, clindamycin.