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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: FEBS J. 2009 Jan;276(1):118–131. doi: 10.1111/j.1742-4658.2008.06760.x

Fig. 6. Hsc70 knockdown inhibits EGF-induced EGFR degradation.

Fig. 6

(A) Equal amounts of proteins from HeLa cell lysates transfected with the indicated siRNA were analyzed by immunoblotting with antibodies against Hsc70 and EEA1. (B) HeLa cells transfected with the indicated siRNAs were incubated with 125I-EGF for 10 minutes at 37°C. The internalized 125I-EGF is expressed as a percentage of the initially bound 125I-EGF. (C) HeLa cells transfected with the indicated siRNAs were allowed to internalize 125I-EGF for 10 minutes and then chased for 1 hour at 37°C. The degraded 125I-EGF is expressed as a percentage of the initially internalized 125I-EGF. Data represent mean ± s.e.m. from 3 independent experiments. The asterisks indicate a statistically significant difference (p < 0.05) from the control siRNA-transfected cells.