Table 2. Immunohistological Evaluation of Liver Sections.
Mouse | Fata | Balloonb | Gradec | Stagec | FASd | LABe | Caspased | Tunelf |
Naive: | ||||||||
005 | 0 | 0 | 0(P0 L0) | 0 | 2 | 0.2 | 0 | 3±2 |
963 | 2 | 1 | 0(P0 L0) | 0 | 1 | 0 | 0 | 8±3 |
992 | 3 | 0 | 2(P0 L2) | 0 | 3 | 0 | 2 m | 8±3 |
996 | 2 | 0 | 2(P0 L2) | 0 | 2 | 0 | 2 m | 11±2 |
986-AAA | 2 | 2 | 3(P0 L3) | 0 | 3 | 0 | 0 | 10±4 |
638-1a Patient serum | 0 | 3 | 2(P0 L2) | 0 | 4 | 0.4 | 2 m | 18±2 |
985-RNA | 3 | 3 | 3(P0 L3) | 0 | 3 | 1.6 | 3 m, h | 50±14 |
975-RNA | 2 | 3 | 3(P0 L3) | 0 | 4 | 4 | 3 m, h | 26±13 |
990-H77c serum | 3 | 2 | 2(P0 L2) | 0 | 4 | 0.4 | 2 m | 50±13 |
The degree of fatty change was scored as 0 (<5%), 1 (6–33%), 2 (34–66%) 3 (>66%).
Hepatocyte ballooning was scored on a scale of 0–4 where 0 is none and 4 is many.
Grade and Stage were scored using the Batts and Ludwig criteria.
FAS and caspase staining was scored semi-quantitatively where 0 = none, 1 = focal weak, 2 = diffuse weak; 3 = diffuse weak and focal strong; 4 = diffuse strong. For caspase staining, m is macrophages and h is hepatocytes.
The lobular apoptotic body count is an average of 5 fields counted at magnification ×100.
The number of Tunel positive nuclei is an average of 15 fields at magnification ×200.