Table 3. Immunohistochemical Evaluation of Liver Sections Stained with Bip/GRP78 or Bax.
Mouse | BiP/GRP78 | BAX | Punctate BAXb |
Cell numbera (Intensity) | Cell numbera (Intensity) | Cell number | |
Naive: | |||
005 | 1 (1) | 1 (1) | 1 |
963 | 2 (2) | 1 (1) | 1 |
992 | 1 (1) | 2 (1) | 2 |
996 | 1 (3-infiltrate) | 0 (0) | 0 |
638-1a Patient serum | 2 (2) | 2 (2) | 2 |
986-AAA | 1 (3-infiltrate) | 2 (1) | 0 |
985-RNA | 3 (1) | 3 (3) | 3 |
975-RNA | 2 (2) | 2 (2) | 3 |
990-Serum | 3 (2) | 2 (2) | 2 |
Staining was scored semi-quantitatively where 0 is no staining 1 (1–15%), 2 (16–30%), and 3 (31–50%), and the intensity of staining was scored on a scale of 0–3, where 0 is no staining and 3 is intense staining.
Activated BAX has a distinct punctate staining which is easily distinguished form inactive BAX, 0 is no staining, 1 (1–5%), 2 (5–10%), and 3 (10–15%). Activated Bax has a uniform intense staining and so no intensity score was given.