Figure 7.
Confocal images of cochlear explant cultures harvested at P0. The explant was exposed to 5 × 109 particles/ml Ad1-cmv-hKCNQ4-G285S for 4 h and cultured for 2 d, and then fixed, mounted, and imaged. A, Confocal image focused at the hair bundle level showing three rows of outer hair cells stained with phalloidin-Alexa546. B, The GFP signal for the same field of hair cells, focused at the cell-body level. C, The same field and focal plane showing KCNQ4 immunoreactivity. D, A merge of A–C with actin in red, GFP in green, and KCNQ4 in blue. Scale bar: (in A) A–D, 10 μm.