Skip to main content
. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Arch Biochem Biophys. 2008 Jul 16;478(1):59–68. doi: 10.1016/j.abb.2008.06.025

Figure 2. Immunochemical properties of CYP4F2 peptide IgG.

Figure 2

Recombinant CYP4F enzymes and human liver microsomes were subjected to SDS-PAGE, electrophoretically transferred to a nitrocellulose membrane, and the membrane immunochemically stained with either anti-CYP4F+ IgG (Panel A) or anti- CYP4F2 peptide IgG (Panel B) as described in Materials and Methods. Lanes 1 and 6, purified CYP4F2 (0.1 µg); lane 2 and 7, purified CYP4F3b (0.1 µg); lanes 3 and 8, CYP4F11-containing Sf9 insect cell lysates (5 µg); lanes 4 and 9, CYP4F12-containing Sf9 insect cell lysates (1 µg); lanes 5 and 10, liver microsomes from subject G (15 µg). The cluster of immunoreactive bands observed in lane 3 stem from the proteolysis of CYP4F11 that occured during preparation of lysates from Sf9 cells expressing this P450.