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. 2009 Jan 20;4:3. doi: 10.1186/1747-1028-4-3

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Copyright © 2009 Cranna and Quinn; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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EcR signaling is required for dmyc expression in wing imaginal discs. (A-D) Discs with control clones to show dmyc-lacZ (w^67c23P{lacW}l(1)G0354^G0354; [109]) enhancer trap activity throughout the cycling cells of the wing pouch, with reduced β-gal antibody staining within the G1 arrested cells of the ZNC (A) β-gal staining (red) (B) GFP (green) positively marks clones (C) Overlay of GFP and β-gal, (D) Merge of GFP, β-gal with DNA (blue) to show the nuclei of apical cells in the wing pouch. (E-H) UAS-EcRAdN clones in the dmyc-lacZ background. (E) β-gal staining (red) (F) GFP (green) marked UAS-EcRAdN clones (G) Overlay of GFP and β-gal. (H) Merged image of GFP, β-gal and DNA (blue).