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Blocking EcR signaling upregulates wg transcription. (A-D) wg-lacZ (P{en1}wgen11; [110]) activity in the wing pouch with control clonal tissue. (A) β-gal (red) (B) GFP (green) marked clonal tissue, (C) Overlay of GFP and β-gal, (D) Merged image of GFP, β-gal and DNA (blue). (E-H) wg-lacZ activity in pouch containing UAS-EcRBdN clones. (E) β-gal (red) (F) GFP (green) (G) Overlay of GFP and β-gal, (H) Merge of GFP, β-gal and DNA (blue). Scale bars indicate 50 μm.
