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. 2008 Dec 16;6:62. doi: 10.1186/1477-7827-6-62

Figure 3.

Figure 3

Regulation of POR and aromatase mRNA and protein levels by FSH. Granulosa cells isolated from immature DES-primed rats were cultured and treated with FSH (30 ng/ml) for the indicated times, and the total RNA or protein was extracted. A: RT-PCR was performed with the specific primer sets for POR, aromatase, and GAPDH. B: Results of real-time PCR are also shown. Data are the mean ± SEM values of at least three independent experiments. *, P < 0.01. C: Western blot analysis was performed with antibodies to POR, aromatase, and GAPDH, using the same lysates.