Figure 5.

Effect of POR knockdown on estrone production and cAMP response of POR and aromatase genes in KGN cells. After transfection with the indicated siRNAs, the culture medium was replaced with that containing 1 mM 8-Br-cAMP and 10 μM androstenedione, and the amount of estrone in the culture medium was detected by EIA 48 h later (B and C). Data are means and SEM values of at least three assays. *, Significant difference in estrone production between KGN cells and KGN cells transfected siRNA for POR#1 or #2 (P < 0.01). The protein expression under these culture conditions at the indicated times is also shown (A and B).