Fig. 6.

Induction of cytokines in vivo. (A and B) Cytokines released into the blood of mice that were bled before (A) and after (B) Sterne challenge were analyzed by using mouse inflammatory and Th1/Th2 cytometric bead array kits. Samples were then analyzed by a FACS CantoII flow cytometer. (C) T cell stimulation. rPA (1 μg/mL) treated and untreated DCs (10⁴ per well) were cultured for 14 h at 37 °C. CD4⁺/CD8⁺ T cells (10⁵ per well) derived from mice that survived the B. anthracis Sterne challenge were cocultured with treated and untreated DCs for 5 days. Cell supernatants were harvested and the cytokine release analyzed by a FACS CantoII flow cytometer. Data are representative of 3 experiments.