Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics |
|---|---|
| L. acidophilus | |
| NCFM | Human intestinal isolate |
| NCK 1838 | NCFM w/ pTRK895 (PA-Ctrlpep) |
| NCK 1839 | NCFM w/ pTRK896 (PA-DCpep) |
| NCK 1895 | NCFM w/ pTRK882 (empty vector) |
| Escherichia coli | |
| MC-1061 | Strr, E. coli transformation host |
| Plasmids | |
| pTRK882 | 4.5-kb, Emr, constitutive expression vector, Ppgm promoter |
| pPAGctrl | 5.7-kb, AmpR source of pagctrl gene |
| pPAGDC | 5.7-kb, AmpR source of pagDC gene, encodes PADC |
| pTRK895 | 6.8-kb, Ermr, pTRK882::pagcon, constitutive expression of PA-ctrlpep |
| pTRK896 | 6.8-kb, Ermr, pTRK882::pagdc, constitutive expression of PA-DCpep |