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. Author manuscript; available in PMC: 2009 Feb 25.
Published in final edited form as: Ann N Y Acad Sci. 2007 Nov;1116:82–99. doi: 10.1196/annals.1402.082

FIGURE 6.

FIGURE 6

BMP does not alter Axin expression or Wnt/β-catenin signaling. Primary chondrocyte cells from Axin2LacZ/+ knockin mice were treated with BMP-2 (100 ng/mL) and cell extracts harvested between 0 and 72 h. β-galactosidase reporter activity was measured (A). C5.18 cells were transfected with the Wnt Topflash reporter and treated with BMP-2 (100 ng/mL) in the presence or absence of Wnt3a. Cell extracts were harvested after 24 h and luciferase activity was measured. The luciferase activity was normalized to renilla luciferase (B).