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. Author manuscript; available in PMC: 2009 Feb 25.
Published in final edited form as: Ann N Y Acad Sci. 2007 Nov;1116:82–99. doi: 10.1196/annals.1402.082

FIGURE 7.

FIGURE 7

Smad3 −/− cells do not exhibit TGF-β-mediated inhibition of Axin expression. Primary chondrocytes from Smad 3−/− mice were isolated and cultured with 5 ng/mL TGF-β treatment for 24 and 48 h. Total RNA was harvested and RT-PCR performed to measure the relative suppression of Axin1 or Axin2 gene expression in TGF-β-treated cultures compared to control cultures after 48 h of treatment (A). Total cell protein extracts were obtained from wild-type and Smad3 −/− chondrocyte cultures treated with TGF-β or control medium and Axin1 protein levels measured by Western blot. β-actin was used as a loading control (B).