Skip to main content
. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Lab Invest. 2009 Jan 12;89(3):290–300. doi: 10.1038/labinvest.2008.163

Figure 1.

Figure 1

A. Shh protein co-distributes with Caveolin-1 in lipid raft fractions. Hepatic stellate cells were lysed in 1% Triton X-100 and subjected to raft isolation by sucrose density gradient ultracentrifugation. Fractions were analyzed by Western blotting for Shh (top panel) and Caveolin-1 (bottom panel). B, C. Shh and Caveolin-1 reciprocally co-precipitate suggesting close physical interaction. Total cell lysates and corresponding immunoprecipitates from beads loaded with control IgG and caveolin-1 antibodies (B) or control IgG and Shh antibodies (C) were analyzed by western blot for caveolin-1 and Shh as indicated.