Fig. 1.

Activation of MPK-1 during the C. elegans life cycle. Synchronized eggs were cultured as described in the methods and worms were collected at L1, L2, L3, L4 larvae and young adult stages. (A) Total protein was extracted and immunoblotted for active phosphorylated MPK-1 (pMPK-1) using a phospho-ERK MAP kinase antibody and α-tubulin as the protein loading control. (B) Quantification of the ratio of pMPK-1 to α-tubulin as measured by densitometry scanning