Figure 6.

An example of data obtained by flow cytometry from a single rat, identifying the percentage of neutrophils to which platelets were bound in blood samples. Blood samples were obtained before pneumonia from venous (A) and arterial (B) catheters and 4 h after instillation of E. coli, also from venous (C) and arterial (D) sites. Cells were labeled with an anti-granulocyte antibody (clone HIS48) followed by phycoerythrin-labeled anti-mouse IgM and a fluorescein isothiocyanate–labeled mouse anti-rat CD42d monoclonal antibody (clone RPM-4) as described in Methods. Flow cytometry was performed by gating on the neutrophils and determining the number of HIS48-positive cells that expressed the platelet marker CD42d (upper right region of each scattergram).