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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: J Immunol. 2009 Mar 1;182(5):2978–2985. doi: 10.4049/jimmunol.0803737

Figure 7. Competitive inhibition of LT-IIb-B5 binding to TLR2 by Pam3CSK4.

Figure 7

Binding of biotinylated LT-IIb-B5 (0.16 μM) to plate-bound TLR2 in the presence of increasing concentrations of unlabeled LT-IIb-B5 or Pam3CSK4. Bound protein was probed with peroxidase-conjugated streptavidin and binding was determined colorimetrically. The data (means ± SD; n = 3) were normalized to the binding activity of labeled LT-IIb-B5 in the presence of 10 mg/ml BSA only (uninhibited control), the mean activity of which was taken as 100. Asterisks indicate significant (p < 0.05) inhibition of binding, and black circles denote significant differences (p < 0.05) between the unlabeled competitors.