Figure 5.

Effect of ASIL1 and Its Interaction with ABA and Auxin on Expression of Seed Maturation Genes in Arabidopsis Seedlings.

Expression of seed maturation genes was analyzed using RNA isolated from either 2-week-old wild-type Col-0 and asil1-1 mutant seedlings that were grown on medium and incubated with 0 (−) or 50 μM (+) ABA for 2 d (A) or wild-type and asil1-1 seeds that had been imbibed for up to 3 h in the presence or absence of 10 μM NAA (B). The mean and sd from qRT-PCR analysis were determined from three biological replicates.

(A) Expression of seed maturation genes in wild-type and asil1-1 seedlings. Total RNA was isolated from 2-week-old plants after exposure to ABA. The levels of various gene transcripts were determined by qRT-PCR using ACT2 mRNA as an internal reference.

(B) Expression of LEC1 and LEC2 in germinating wild-type and asil1-1 seeds. Total RNA was isolated from desiccated seeds or seeds that had been imbibed with the presence of NAA. Real-time RT-PCR was used to examine the levels of LEC1 and LEC2 transcripts with Ef-1α as a reference gene.