Figure 6.

Accumulation of Seed Storage Reserves in asil1 Seedlings.

(A) TAG in wild-type (Col-0) and asil1-1 and asil1-2 mutant seedlings. Total lipids were extracted from 3-week-old seedlings grown in soil and treated with 0 (−) or 50 μM (+) ABA for 24 h before harvest. An equivalent amount of extracts from wild-type and asil1 seedlings were separated by TLC and stained with sulphuric acid. Lipid extracts from wild-type seeds were used as a control, and glyceryl trilinoleate (10 μg) was used as a standard (Std). The position of the seed-specific TAG is indicated by an arrow.

(B) Fatty acid composition of TAG fraction from wild-type seeds and asil1 seedlings treated with ABA. TAG fraction was separated by TLC, and the fatty acid composition was analyzed on a gas chromatograph and expressed as a percentage of the total TAG fraction. Results represent the average and sd from three biological replicates.

(C) Accumulation of 2S albumin in asil1 seedlings. Protein extracts (20 μg) from 3-week-old wild-type and asil1-1 and asil1-2 mutant seedlings grown in soil and treated with 0 (−) or 50 μM (+) ABA for 24 h were resolved by SDS-PAGE and analyzed by either Coomassie blue staining (left) or immunoblotting with polyclonal antibody raised against 2S albumin (right). Protein extracts (0.5 μg) from wild-type seeds were used as a control. A portion of the membrane blot is shown (right).