Figure 4.
Accumulation of Disinapoyl Spermidine and Expression of SDT in Arabidopsis during Seed Germination.
(A) Expression of SDT in different organs of Arabidopsis. RNA was extracted from different organs of mature plants. qRT-PCR was performed with gene-specific primers, and ef1α was used a constitutive control. The data represent the mean value (±sd) of two independent biological replicates.
(B) Siliques harvested at different stages of development.
(C) Contents of 4′-O-hexosyl-N1,N8-di(sinapoyl)-spermidine (S1) and N1,N8-di(sinapoyl)-spermidine (S2) in siliques harvested at the different developmental stages indicated in (B).
(D) Relative expression levels of SDT in siliques harvested at the different developmental stages indicated in (B). The data represent the mean value (±sd) of two independent biological replicates.
(E) GUS staining of seeds in an untransformed wild-type silique.
(F) GUS staining (blue color) of seeds carrying the SDT promoter:GUS fusion.