Table 2.
Kinetics of Recombinant At2g25150 on Different Acyl Donors and Acceptors
| Relative Activity (%)a | kcat (s−1) | Km (μM) | |
|---|---|---|---|
| Acyl donorb | |||
| Coumaroyl-CoA | 100 | 3.4 ± 0.5 | 10.6 ± 1.5 |
| Sinapoyl-CoA | NDc | ||
| Caffeoyl-CoA | ND | ||
| Feruloyl-CoA | ND | ||
| Acyl acceptord | |||
| Spermidine | 100 | 3.1 ± 0.4 | 52.7 ± 8.9 |
| Putrescine | ND | ||
| Cadaverine | ND | ||
| Spermine | ND | ||
| Agmatine | ND | ||
| Tyramine | ND |
Specific activities were determined under the conditions described in Methods. Acyl donors and acceptors were used at final concentrations of 60 and 200 μM, respectively, when determining the Km for the other substrate types. The specific activity with coumaroyl-CoA and spermidine (52.4 nkat mg−1) was taken to be 100%. The relative activities were determined from the product peak integrals using coumaric acid as a standard. All the reactions were run in duplicate, and each experiment was repeated twice. The data represent the mean value (±sd).
The reactions were performed using spermidine as the acyl acceptor.
Not detected.
The reactions were performed using sinapoyl-CoA as the acyl donor.