Figure 5.

Evidence of aTI Activity in Multiple Arabidopsis Genes.

(A) Three Arabidopsis loci were tested in vitro for evidence of ATI activity based on features identified within the annotated 5′ UTR sequence. Constructions were designed to include (UTR) or omit (ATG) the 5′ UTR sequence (250 bp in each case). An additional 444 bp beyond the ATG was included in each construction. IVTT reactions with wheat germ extracts were fractionated by PAGE using similar conditions to those in Figures 1 to 3.

(B) At Polγ1 and Polγ2 gene constructions with and without the 5′ UTR sequence were subjected to IVTT to test for evidence of aTI activity at the conserved CTG initiator site.