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. 2009 Jan;21(1):90–105. doi: 10.1105/tpc.108.061812

Figure 1.

Figure 1.

EDE1 Is Essential for Seed Development.

(A) to (D) Confocal micrographs of Feulgen- stained seeds of the wild type (A) and ede1-1 mutant ([B] to [D]) at 6 DAP. Note the NCDs that invade the central vacuole (CV) of the endosperm. Only few enlarged NCDs are present in the ede1-1 mutant when compared with the wild type. The range of ede1-1 phenotypes is also shown: mild (B), moderate (C), and extreme (D). EM, embryo; CE, chalazal endosperm. Bars = 20 μm.

(E) Developmental stages of the wild type (top row) and of ede1-1 mutant endosperm (bottom row). Whole mounts of cleared seeds were observed at different developmental stages (0 to 8 DAP) using differential interference contrast optics. The enlarged nuclei in the mutant are marked by asterisks. Bar = 20 μm (0 to 2 DAP) and 50 μm (4 to 8 DAP).