Figure 7.

GFP-EDE1 Localizes to Microtubules during Mitosis.

Living tobacco BY-2 suspension cells transformed with EDE1 promoter:GFP-EDE1. Bar = 10 μm.

(A) GFP-EDE1 accumulates in nuclear caps from which radial microtubules emanate in premitotic cells.

(B) During mitosis, fluorescence accumulates in metaphase spindle and spindle poles.

(C) and (D) Labeling of the kinetochore microtubules in anaphase, together with labeling around the spindle poles.

(E) GFP-EDE1 strongly associated with midzone microtubules from which the early columnar phragmoplast develops.

(F) Fluorescence remains associated with the phragmoplast throughout cell plate formation.