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Excess Nickel Mimics Copper in Regulating the Expression of miR398.
(A) RT-PCR analysis of CSD mRNAs in seedlings grown for 3 weeks on MS medium containing the indicated concentrations of copper and nickel. The number of PCR cycles was 22 for CSD2, 25 for miR398b/c, and 23 for Actin (control).
(B) Immunoblot analysis of CSDs and cytochrome f in the seedlings used in (A).
