Figure 7.

SHB1 Is Expressed in Endosperm and Embryo.

Expression of ProSHB1:GUS in eight-day old seedling (A), inflorescence (B), flower (C), mature pollen (D), in vitro germinating pollen (E), and unfertilized ovule (F). In situ hybridization of Ws with SHB1 antisense probe at early globular stage (G), early heart stage (H), heart stage (I), torpedo stage (J), and late torpedo stage (K) or with SHB1 sense probe at torpedo stage (L). A, anther; C, cotyledon; Ch, chalazal end; CZE, chalazal endosperm; En, endosperm; EP, embryo proper; ES, embryo sac; F, filament; H, hypocotyl; MCE, micropylar endosperm; Ov, ovule; P, petal; PEN, peripheral endosperm; PG, pollen grain; PT, pollen tube; R, embryonic root; RL, rosette leaf; S, suspensor; Se, sepal; Sti, stigma; Sty, style. Bars = 1 mm for (A) to (C), 25 μm for (D) to (F), and 50 μm for (G) to (L).