Figure 8.

SHB1 Associates with MINI3 and IKU2 Promoters in Vivo.

(A) Schematic diagram of the MINI3 and IKU2 loci and the five amplicons (i.e., M1, M2, M3, IK1, and IK2) used for ChIP analysis.

(B) and (C) Enrichment of particular MINI3 and IKU2 chromatin regions with anti-SHB1 antibody in wild-type plants (B) or with anti-GFP antibody in SHB1:GFP transgenic plants (C) as detected by real-time PCR analysis. Preimmune serum was used as mock control. Means were calculated from three biological samples, and each biological sample was examined with three PCR technical replicates. The calculated se includes technical error and biological error.