Table 1.

Potential RBP50 Phloem-Interacting Proteins Identified by Co-IP Experiments

Interacting Protein	Protein Identity	Molecular Mass (kD)
IP1	Myosin heavy chain–like protein	149
IP2	Heat shock protein–related protein, Gly dehydrogenase	113, 113
IP3	PP1	96
IP4	Expressed protein	89
IP5	Phosphoinositide-specific phospholipase–like protein	68
IP6	Glutathione-regulated potassium efflux system protein	67
IP7	Glycosyl hydrolase family 1 protein	65
IP8	RBP50	50
IP9	Expressed protein	46
IP10	Putative ATP binding protein	44
IP11	GTP binding protein	39
IP12	Shikimate kinase precursor	33
IP13	RBP50 cleavage product, Cys proteinase inhibitor	26
IP14	Csf-2 related protein	19
IP15	PP16-1	17
IP16	PP16-2	16
IP17	Cys proteinase inhibitor like protein, putative protein	12

RBP50–RNP complexes were coimmunoprecipitated from total phloem sap using purified anti-RBP50 antibody. Proteins were separated by SDS-PAGE, and individual bands were excised from the gel for LC-MS/MS analysis and protein identification.