Table 3.
Phloem Transcript Identity | Transcript Abundance |
---|---|
PP16-1 | 15 |
GAIP | 9 |
SCARECROW-LIKE (SCL14P) | 7 |
SHOOT MERISTEMLESS (STMP) | 5 |
ETHYLENE RESPONSE FACTOR (ERFP) | 5 |
Myb (MybP) | 4 |
Total pumpkin phloem sap was used to coimmunopurify RNP complexes. mRNA contained within these RNP complexes was extracted, cloned, and sequenced. Note that in naming these transcripts, the letter P is used to designate them as being contained within the phloem translocation stream. Transcript abundance represents the cloning results from five independent co-IP experiments in which the isolated RNA was pooled for analysis. Data presented represent the number of times each transcript was identified from all 35 amplified colonies.