Figure 1.

Whole Plant and Nodulation Phenotype of L. japonicus Wild-Type, pir1-1, nap1-1, and pir1-1 nap1-1 Mutant Plants.

(A) Wild-type L. japonicus.

(B) pir1-1.

(C) nap1-1.

(D) and (E) pir1-1 nap1-1 double mutant.

(F) pir1-1 Fix⁻ bump (left) and one of the rare oversized red nitrogen-fixing nodules (right).

(G) nap1-1 Fix⁻ nodules.

(H) One of the rare nap1-1 oversized red nitrogen-fixing nodules.

(I) Size comparison between L. japonicus wild-type (left) and an oversized pir1-1 nodule (right).

Bars = 1 cm in (A) to (E) and 2 mm in (F) to (I).

(J) Nodule numbers on 6-week-old nap1-1, pir1-1, pir1-1 nap1-1, and wild-type plants inoculated with M. loti NZP2235. Black columns: mature, red nodules (red); gray columns: white immature, Fix⁻ nodules (bump). Bars indicate 95% confidence intervals.