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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: J Immunol. 2009 Mar 1;182(5):2786–2794. doi: 10.4049/jimmunol.0803484

Figure 1. OT-I cells lacking IL-12 and IFN-α/β receptors can develop cytolytic activity in response to Ag, B7-1 and IL-21.

Figure 1

Purified, naïve (CD44lo) (A) OT-I.WT or (B) OT-I.DKO cells were placed in culture with aAPC bearing H-2Kb/OVA257–264 and B7-1 on the surface, as previously described (8). Cultures received no cytokine, IL-12 (2U/ml), IFN-a (1500U/ml) or IL-21 (100ng/ml). Three days later cytolytic activity was determined in triplicate in a standard 4hr 51Cr-release assay using OVA257-264-peptide pulsed EL-4 targets. Results are representative of three independent experiments.