Figure 3. TRP-2-specific CD8⁺ T cells can be activated in vivo and generate effector function.

A,B. On day 0, CFSE-labelled Thy1.1⁺ LN cells from TCR Tg mice were adoptively transferred into C57BL/6 mice. On day 1, mice were vaccinated subcutaneously with unpulsed (DC-CTRL) or peptide-pulsed (DC-TRP-2) bone marrow-derived dendritic cells. Five days after vaccination, vaccine draining LN cells were analyzed by gating on adoptively-transferred Thy1.1⁺ CD8⁺ T cells and assessing dilution of CFSE and CD44 (A) or IFN-γ expression (B). Data are presented for a single animal where identical results were obtained for 3 animals per group; the experiment was performed twice.

C. Lysis of TRP-2-pulsed target cells was assessed by injecting mice with CFSE-labelled splenocytes cells after vaccination with dendritic cell vaccines. The frequency of CFSE^LOW (unpulsed) and CFSE^HI (TRP-2-pulsed) cells were analyzed in vaccine-draining LNs of mice transferred with TCR Tg cells (W/T-ACT) and untransferred mice (W/T). The percent specific lysis is presented in each panel. Data are presented for a single animal where identical results were obtained for 5 animals per group; the experiment was performed four times.