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. Author manuscript; available in PMC: 2009 Dec 15.
Published in final edited form as: Free Radic Biol Med. 2008 Sep 17;45(12):1695–1704. doi: 10.1016/j.freeradbiomed.2008.09.002

Figure 3. The radiation-induced pro-inflammatory response is regulated, in part, by NF-κB and AP-1.

Figure 3

A and B, BV-2 cells were treated with either SP 600125 (SP) or 6-Amino-4-(4-phenoxyphenylethylamino) quinazoline (Q) for 1 h, and then irradiated with a single dose of 10 Gy. Nuclear extracts were collected 1 h post-irradiation and subjected to EMSA. Data are presented as fold changes in DNA-binding of NF-κB (A) and AP-1 (B) compared to sham-irradiated cells. Mean ± S.E.M; *, p< 0.05 vs. sham-irradiated cells; #, p< 0.05 vs. 10 Gy; n.s, non-significant vs. 10 Gy; n = 3. C, BV-2 cells were treated with the inhibitors as above. TNFα (black bars) and IL-1β (dotted bars) gene expression was measured using real-time qPCR 1 h and 24 h post-irradiation, respectively. Cox-2 (checked bars) protein expression was measured 7 h post-irradiation. Data are presented as fold changes compared to sham-irradiated cells. Mean ± S.E.M; *, p< 0.05 vs. sham-irradiated cells; #, p< 0.05 vs. 10 Gy; n.s, non-significant vs. 10 Gy, n = 3.