Skip to main content
. Author manuscript; available in PMC: 2009 Jul 22.
Published in final edited form as: Nature. 2008 Dec 10;457(7228):490–494. doi: 10.1038/nature07547

Figure 1.

Figure 1

Fetal bone (fb) cells can initiate an ectopic HSC niche. a, Ectopic bone formed by GFP-labelled, 14.5 dpc fb cells 32 days after subrenal capsule transplant (scale bar = 500 μM). b, Representative section of graft stained with pentachrome (yellow=osteoid, greenish blue= cartilage), cartilaginous region in black circle (scale bar = 100 μM). c, Safranin-O stain of adjacent section, red-staining cartilage matrix in yellow circle (scale bar = 200 μM). d, Alizarin Red stain for calcified tissue (scale bar = 500 μM). e, Time course study of hematopoietic components during ectopic niche formation. Hematoxylin-and-Eosin staining (upper panel, scale bar = 100 μM), representative FACS profiles of LT-HSC (CD45+Lineage-c-Kit+Sca1+CD150+) frequency that were pre-gated for live, CD45+lineage- cells (lower panel). Days after transplantation are indicated; (n=4) for each time point.