FIG. 3.

Relative mRNA levels of hrpS, hrpL, dspE, hrpA, hrpN, and rsmB of D. dadantii 3937 in MM supplemented with 100 μM PCA compared to mRNA levels in MM without PCA as determined by qRT-PCR. There is no significant difference between MM and MM supplemented with PCA for gene rsmB (P = 0.928), but levels of gene expression of hrpS, hrpL, dspE, hrpA, and hrpN are significantly different between MM and MM supplemented with 100 μM PCA (P < 0.001). Three replicates were used in this experiment. The P value was calculated with the Relative Expression Software Tool as described by Pfaffl et al. (15).