TABLE 3.

Expression of T3SS genes hrpA, hrpN, hrpS, and hrpL of D. dadantii 3937 in MM and MM supplemented with 100 μM PCA

Reporter plasmid	Avg MFI ± SD for growth in the indicated mediuma at:
	12 h		24 h
	MM	MMPCA	MM	MMPCA
phrpA	64.3 ± 0.9	10.3 ± 1.3*	150.8 ± 4.4	18.5 ± 3.5*
phrpN	39.8 ± 5.8	6.8 ± 0.8*	133.3 ± 3.2	11.3 ± 3.4*
phrpS	72.7 ± 11.3	37.9 ± 1.3*	95.0 ± 17.7	43.6 ± 2.6*
phrpL	12.8 ± 0.1	7.8 ± 0.1*	27.2 ± 1.0	11.1 ± 3.3*
pmrp	113.0 ± 7.7	124.1 ± 2.7	93.4 ± 2.6	98.9 ± 1.0
pPROBE-AT	2.1 ± 0.1	2.2 ± 0.2	13.4 ± 8.4	14.0 ± 10.1

^aThe promoter activities were compared at 12 and 24 h of bacterial growth in PCA. GFP mean fluorescence intensity (MFI) was determined for gated populations of bacterial cells by flow cytometry. Values are representative of two experiments, and three replicates were used for each experiment. Asterisks indicate statistically significant differences in GFP intensity between bacterial cells grown in MM and MM supplemented with 100 μM PCA (MMPCA) (P < 0.01, Student's t test).