FIG. 1.
Tape-FISH for detection of Salmonella strains in mixed culture from tomato surfaces. Tomatoes were spiked with a mixture of S. enterica serovar Typhimurium (107 CFU cm−2) and R. glutinis (106 CFU cm−2) and then sampled with adhesive tape after drying. Tapes were hybridized for 30 min with a combination of probes targeting Salmonella cells (Sal3/Salm-63 cocktail, green label) and eukaryotic cells (EUK 516, red label). These results demonstrate the utility of tape-FISH for simultaneous visualization of the distribution and interactions between multiple phylotypes occurring together on produce surfaces.