TABLE 2.
Genes and primers used for MLST
| Gene | Enzyme function | Positiona | Primer | Sequence (5′-3′) | Amplicon size (bp) |
|---|---|---|---|---|---|
| gyrB | Gyrase, β subunit | 4101-6059 | gyrB-1 | CTTCGGTTGTTAATGCTTTGTC | 674 |
| gyrB-2 | CAACTTGGTTTTTGTCTGCC | ||||
| g6pd | Glucose-6-phosphate dehydrogenase | 122134-123606 | g6pd-1 | TTATATGTCTGTTGCTCCTCGT | 669 |
| g6pd-2 | CCGGTTCTGATGTAAAAAGG | ||||
| pgm | Phosphoglucomutase | 354671-356371 | pgm-1 | ATATCTGCCGAAGTGCTAAGAG | 654 |
| pgm-2 | AGCAGCAATTTGATTTCCAG | ||||
| ddl | d-Ala-d-Ala ligase | 634208-635341 | ddl-1 | GGGAATTCATCTGAACACGA | 675 |
| ddl-2 | GATTGGGCTGGTAATGACAC | ||||
| dnaE | DNA polymerase III, α subunit | 934193-937075 | dnaE-1 | CGTATATAGAGCGCTTTGCC | 714 |
| dnaE-2 | CGTTCTTATCGCGAGTTGTAC | ||||
| purK | Phosphoribosylamino-imidazole carboxylase | 1071235-1072353 | purK-1 | TGGTTATCATGTTGGTATTTTGG | 597 |
| purK-2 | GAAGCAGGAGCATAGGAAAGA | ||||
| rpoB | RNA polymerase, β subunit | 1282812-1286405 | rpoB-1 | CGATATTCTCCTTTCTCCAATG | 665 |
| rpoB-2 | CTTTAGCGATCTGTTCCAATG | ||||
| recP | Transketolase | 1393609-1395648 | recP-1 | AGCGACAAACCATCCTTTATC | |
| recP-2 | CGACAGCTAAGGAATCATGAG | 676 |
a
Numbers denote the positions of the first and last bases of the gene on the O. oeni PSU-I chromosome.