TABLE 2.
Templates, primers, and amplified fragments for the construction of chimeric mutants by PCR
| Template(s) | Primers | Amplified fragmenta | Size (bp) |
|---|---|---|---|
| pDr | Dr-FP, CTT-RP | CTTDr | 1,934 |
| pDg | Dg-FP, H2 | NADg | 1,571 |
| pDr | H1, Dr-RP | CDr | 643 |
| NADg, CDr | Dg-FP, Dr-RP | GGR | 2,188 |
| pDg | H2, Dg-RP | CDg | 447 |
| pDr | Dr-FP, H1 | NADr | 1,527 |
| NADr, CDg | Dr-FP, Dg-RP | RRG | 1,948 |
| pDg | Dr-FP, H4 | NDr | 517 |
| pDr | H3, Dg-RP | ACDg | 1,457 |
| NDr, ACDg | Dr-FP, Dg-RP | RGG | 1,948 |
| pDg | Dg-FP, H4 | NDg | 566 |
| pDr | H3, Dr-RP | ACDr | 1,653 |
| NDg, ACDr | Dg-FP, Dr-RP | GRR | 2,193 |
| pDg | Dg-FP, DgCT-RP | CTDg | 1,601 |
| pDg | DgNT-FP, Dg-RP | NTDg | 1,521 |
a
The N, A, and C domains and combinations of these domains (e.g., NA) are identified by the subscripted abbreviations used in the text for D. geothermalis (Dg) and D. radiodurans (Dr).