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. 2008 Dec 29;75(5):1437–1444. doi: 10.1128/AEM.01781-08

FIG. 1.

FIG. 1.

Epifluorescence micrographs of bacteria on a thin section in the tentacle region of a L. pertusa polyp (red coral color variety from station 3). (a) Overview of a cross-sectioned tentacle. The image is an overlay of coral tissue autofluorescence (green), DAPI signals of coral cell nuclei (blue), and hybridization signals of probes EUB338 I to III with Cy3 TSA (red). (b and c) Micrograph detail showing DAPI (b) and Cy3 (c) signals of bacterial aggregates on the nematocyst batteries of the tentacle ectoderm. (d) Single flask-shaped bacterial cells. *, frame of micrograph detail (panels b and c); nb, nematocyst battery; nu, coral cell nuclei; s, nematocyst stylet. Arrows in panels b and c denote corresponding DAPI and Cy3 signals of selected bacterial aggregates. Scale bars: 100 μm (a), 20 μm (b and c), and 5 μm (d).