UPGMA phylogram of cob barcode sequences from cultured and Mirror Lake dinoflagellate clones. Bootstrap values, analyzed based on 500 replications, were indicated for major clades by branch thickness: thickest line, >95%; second-thickest line, 75 to 95%; thin line, <75%. Dinoflagellate cob clones recovered from Mirror Lake are in bold type. Note that Ceratium and a novel lineage that was affiliated between Crypthecodinium and Symbiodinium occurred and dominance shifted over time. The scale bar indicates the substitution rate per nucleotide (0.1).