FIG. 2.

Fluid convection through 24-h-old S. epidermidis and A. pleuropneumoniae biofilms cultured in centrifugal filter devices. Devices were subjected to low-speed centrifugation, and the cumulative flowthrough volume was measured after increasing amounts of time. Blank filters were inoculated with sterile broth and incubated alongside the biofilm-containing filters. (A) Fluid convection through S. epidermidis NJ9709 biofilms cultured in the absence or presence of 20 μg of dispersin B (DspB) ml⁻¹. (B) Fluid convection through S. epidermidis 9142 biofilms cultured in the absence or presence of 20 μg ml⁻¹ of dispersin B and through S. epidermidis M10 (PNAG mutant) biofilms. (C) Fluid convection through A. pleuropneumoniae IA5 biofilms cultured in the absence or presence of 20 μg of dispersin B ml⁻¹ and through A. pleuropneumoniae EI1001 (PNAG mutant) biofilms. Values show means and ranges of flowthrough volumes for duplicate centrifugal filter devices.