FIG. 5.

Transport of CPC and Allura red dye through A. pleuropneumoniae and S. epidermidis biofilms. (A) Transport of CPC through A. pleuropneumoniae biofilms cultured in centrifugal filter devices. Values show the concentration of CPC in the flowthrough volume for a control filter inoculated with sterile MHB (Blank filter), a filter inoculated with wild-type strain IA5, a filter inoculated with PNAG mutant strain EI1001, and a filter inoculated with IA5 and cultured in the presence of 20 μg of dispersin B ml⁻¹ (IA5 + DspB). (B) Elution of CPC from A. pleuropneumoniae IA5 and S. epidermidis NJ9709 biofilms in centrifugal filter devices. Biofilms were perfused by centrifugation with 0.03% CPC and then with water. The values show the concentrations of CPC in the flowthrough volume after a third perfusion with water or 1 M NaCl. (C) S. epidermidis NJ9709 biofilms cultured in centrifugal filter devices were perfused with water or CPC, and the rate of water convection was then measured as described for Fig. 1. (D) Transport of Allura red dye through S. epidermidis NJ9709 biofilms cultured in centrifugal filter devices or as colony biofilms. Biofilms were perfused with water or CPC prior to the addition of the Allura red dye. Values show the absorbance of the flowthrough volume (for the centrifugal filter assay) or the absorbance of an agarose plug containing the absorbed dye (for the colony biofilm assay). In all panels, values show means and ranges of the results of duplicate assays.