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PlyC minimal contact time for 100% disinfection. One microgram of PlyC was mixed with 1 × 107 CFU/ml of S. equi 9528 (Smr) in a final volume of 1 ml of PBS, and 100-μl aliquots were serially diluted and plated at 0, 5, 10, 15, and 20 min on blood agar plates containing 200 μg/ml streptomycin to estimate residual CFU. The control contained no PlyC. Error bars show standard deviations of triplicates.
